pCut Plasmid Toolkit
Depositing Lab: Aindrila Mukhopadhyay
This 27 plasmid Cas9-based toolkit is used to quickly institute genetic changes in S. cerevisiae for optimizing heterologous gene expression. This kit contains Cas9-sgRNA (pCut) plasmids for integrating into 23 well-characterized loci, across all 16 yeast chromosomes. The gRNA sequences can be easily exchanged to target additional loci and enable deletion or replacement of genes.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.