Addgene - 884 pGEX2T PTEN G129E Plasmid Data

Browse > William Sellers > Ramaswamy et al > 884 pGEX2T PTEN G129E

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NCBI: PTEN

PTEN antibodies

This is commonly
requested with

819 pGEX2T PTEN C124S

823 pGEX2T PTEN G129R

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Plasmid 10747: 884 pGEX2T PTEN G129E

Gene/insert name:		PTEN
Insert size (bp):		1200
Gene/insert aliases:		PTEN, BZS, MHAM, TEP1, MMAC1, PTEN1, 10q23del, MGC11227
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		G129E
Fusion proteins or tags:		GST
Terminal:		N terminal on backbone
Vector backbone:		pGEX-2T (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		4948
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		pGEX-5' (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Principal Investigator:		William Sellers
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Ramaswamy S et al. (Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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