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Mouse Toronto KnockOut (mTKO) CRISPR Library
(Pooled Libraries #159392, #159393)

  • Purpose

    Mouse lentiviral genome-wide CRISPR library targets protein-coding genes with ~5 gRNAs/gene.

  • Vector Backbone

    #159392: 1 vector system in lentiCRISPRv2 backbone - expresses Cas9
    #159393: 2 vector system in pLCKO2 backbone - does not express Cas9

Ordering

1 vector system (lentiCRISPRv2)
This backbone encodes Cas9.

Item Catalog # Description Quantity Price (USD)
Pooled Library 159392 gRNA pooled library in lentiCRISPRv2 1 $500 Add to Cart
Available to Academic and Nonprofits Only

2 vector system (pLCKO2)
A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lenti-Cas9-2A-Blast (Addgene #73310) or with cell lines already expressing Cas9.

Item Catalog # Description Quantity Price (USD)
Pooled Library 159393 gRNA pooled library in pLCKO2 1 $500 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Genes targeted
    19,463
  • gRNAs
    94,528
  • Controls
    418 control gRNAs targeting EGFP, LacZ, and Luciferase
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

The depositing lab recommends using Bayestian Analysis of Gene Essentiality (BAGEL) to analyze fold changes.

MAGeCK/MAGeCK-VISPR algorithms can also be used for analysis.

The DrugZ algorithm is recommended for drug screens.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Toronto KnockOut CRISPR library (mTKO) was a gift from Jason Moffat (Addgene #)
  • For your References section:

    Functional genomic landscape of cancer-intrinsic evasion of killing by T cells. Lawson KA, Sousa CM, Zhang X, Kim E, Akthar R, Caumanns JJ, Yao Y, Mikolajewicz N, Ross C, Brown KR, Zid AA, Fan ZP, Hui S, Krall JA, Simons DM, Slater CJ, De Jesus V, Tang L, Singh R, Goldford JE, Martin S, Huang Q, Francis EA, Habsid A, Climie R, Tieu D, Wei J, Li R, Tong AHY, Aregger M, Chan KS, Han H, Wang X, Mero P, Brumell JH, Finelli A, Ailles L, Bader G, Smolen GA, Kingsbury GA, Hart T, Kung C, Moffat J. Nature. 2020 Oct;586(7827):120-12. doi: 10.1038/s41586-020-2746-2. PubMed 32968282