Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 81 - 100 of 565 results

  1. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

    Enlarge

  2. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107576
    Purpose
    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
    107576-plasmid-map-sequence-id-214314

    Enlarge

  3. pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107577
    Purpose
    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    gapdh promoter - B. megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
    107577-plasmid-map-sequence-id-209152

    Enlarge

  4. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107578
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fbp promoter Bacillus megaterium DSM319
    Availability
    Academic Institutions and Nonprofits only
    107578-plasmid-map-sequence-id-209151

    Enlarge

  6. pSPYngK-hp

    Plasmid
    #48118
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of YngK
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48118-plasmid-map-sequence-id-69944

    Enlarge

  7. pSPVpr-hp

    Plasmid
    #48119
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of Vpr
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48119-plasmid-map-sequence-id-69945

    Enlarge

  8. pSPNprM-hp

    Plasmid
    #48120
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of NprM
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48120-plasmid-map-sequence-id-69946

    Enlarge

  9. pSPPac-hp

    Plasmid
    #48123
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of Pac
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48123-plasmid-map-sequence-id-69950

    Enlarge

  10. pHBintT

    Plasmid
    #48134
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48134-plasmid-map-sequence-id-69938

    Enlarge

  11. pHBintN

    Plasmid
    #48135
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48135-plasmid-map-sequence-id-69940

    Enlarge

  12. pHBintE

    Plasmid
    #48136
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48136-plasmid-map-sequence-id-69942

    Enlarge

  14. pET28a-baPrs-hdNadV

    Plasmid
    #91950
    Purpose
    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi
    Depositor
    George Cătălin Marinescu
    Article
    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10
    Inserts
    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Leucine 135 to Isoleucine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    91950-plasmid-map-sequence-id-194125

    Enlarge

  18. pSPYngK+-hp

    Plasmid
    #48121
    Purpose
    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    codon optimized signal peptide of YngK
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48121-plasmid-map-sequence-id-69947

    Enlarge

Showing: 81 - 100 of 565 results