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Addgene
Showing: 101 - 120 of 180 results
  1. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid
    #176276
    Purpose
    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Insert
    MCS-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin I
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid
    #176277
    Purpose
    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Insert
    MCS-P2A-mCherry
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin I
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Availability
    Academic Institutions and Nonprofits only
  4. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Availability
    Academic Institutions and Nonprofits only
  5. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Availability
    Academic Institutions and Nonprofits only
  6. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and synthetic Probasin ARRx2
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-EF1a-Flpo (AAV1)

    Viral Prep
    #55637-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-EF1a-Flpo (AAV2)

    Viral Prep
    #55637-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV-CAG-FlpO (AAV1)

    Viral Prep
    #183412-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-FlpO (#183412). In addition to the viral particles, you will also receive purified pAAV-CAG-FlpO plasmid DNA. CAG-driven expression of the recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Promoter
    CAG
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV-EF1a-Flpo (AAV Retrograde)

    Viral Prep
    #55637-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV-CAG-FlpO (AAV Retrograde)

    Viral Prep
    #183412-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-FlpO (#183412). In addition to the viral particles, you will also receive purified pAAV-CAG-FlpO plasmid DNA. CAG-driven expression of the recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Promoter
    CAG
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  12. AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)

    Viral Prep
    #51669-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    Syn
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  13. pAAV-EF1a-mCherry-IRES-Flpo (AAV1)

    Viral Prep
    #55634-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. EF1a-driven mCherry and bicistronic Flp recombinase expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    EF1a and Ef1a/IRES
    Tags
    mCherry
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 180 results