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Targeting cells with single vectors using multiple-feature Boolean logic.
Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K
Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 2014 Jun 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
55632pAAV-Ef1a-mCherry-IRES-CreExpresses Cre in Mammalian Cells
55633pAAV-EF1a-mCherry-IRES-DreExpresses Dre in Mammalian Cells
55634pAAV-EF1a-mCherry-IRES-FlpoExpresses Flpo in Mammalian Cells
55635pAAV-EF1a-sCreExpresses sCre in Mammalian Cells
55636pAAV-EF1a-CreExpresses Cre in Mammalian Cells
55637pAAV-EF1a-FlpoExpresses Flpo in Mammalian Cells
55638pAAV-EF1a-vCreExpresses vCre in Mammalian Cells
55639pAAV-Ef1a-fDIO hChR2(H134R)-EYFPFlp-Dependent ChR2-EYFP
55640pAAV-Ef1a-dDIO hChR2(H134R)-EYFPDre-Dependent ChR2-EYFP
55641pAAV-Ef1a-fDIO EYFPFlp-Dependent EYFP
55642pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFPsCre-Dependent ChR2-EYFP
55643pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFPvCre-Dependent ChR2-EYFP
55644pAAV-nEF Con/Fon hChR2(H134R)-EYFPCre-on/Flp-on ChR2-EYFP under the short Ef1a promoter
55645pAAV-hSyn Con/Fon hChR2(H134R)-EYFPCre-on/Flp-on ChR2-EYFP under the Synapsin promoter
55646pAAV-hSyn Con/Foff hChR2(H134R)-EYFP Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter
55647pAAV-nEF Con/Foff hChR2(H134R)-EYFPCre-on/Flp-off ChR2-EYFP under the short Ef1a promoter
55648pAAV-hSyn Coff/Fon hChR2(H134R)-EYFPCre-off/Flp-on ChR2-EYFP under the Synapsin promoter
55649pAAV-hnEF Coff/Fon hChR2(H134R)-EYFPCre-off/Flp-on ChR2-EYFP under the short Ef1a promoter
55650pAAV-hSyn Con/Fon EYFPCre-on/Flp-on EYFP under the Synapsin promoter
55651pAAV-hSyn Con/Foff EYFPCre-on/Flp-off EYFP under the Synapsin promoter
55652pAAV-hSyn Coff/FonEYFPCre-off/Flp-on EYFP under the Synapsin promoter

Antibodies from Article