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Addgene
Showing: 1 - 20 of 121 results
  1. pENTR223 CD147, open

    Plasmid
    #149720
    Purpose
    for Gateway cloning of CD147 into destination vector
    Insert
    CD147 (BSG Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    wt, without stop codon
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. AAVS1-Hb9-CD14

    Plasmid
    #204344
    Purpose
    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
    Depositor
    Insert
    human CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hb9 (Mnx1) 9kb promoter fragment
    Availability
    Academic Institutions and Nonprofits only
  3. U5m

    Plasmid
    #121018
    Purpose
    MoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Insert
    BCD14
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pBOB-VE-cadherin-tdTomato

    Plasmid
    #118733
    Purpose
    To express VE-cadherin tagged with tdTomato on C-terminus
    Depositor
    Insert
    Cadherin 5 (CD144) (CDH5 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Expression
    Mutation
    I503T (Common SNP-see comments)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Availability
    Academic Institutions and Nonprofits only
  6. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Availability
    Academic Institutions and Nonprofits only
  7. R777-E155 Hs.PDGFRA

    Plasmid
    #70439
    Purpose
    Gateway ORF clone of human PDGFRA [NM_006206.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    PDGFRA (PDGFRA Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. R777-E156 Hs.PDGFRA-nostop

    Plasmid
    #70440
    Purpose
    Gateway ORF clone of human PDGFRA [NM_006206.4] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    PDGFRA (PDGFRA Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 121 results