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Addgene
Showing: 1 - 20 of 11277 results
  1. pJM483

    Plasmid
    #52987
    Purpose
    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor
    Depositor
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Availability
    Academic Institutions and Nonprofits only
  2. pSP64-mHCN2

    Plasmid
    #53060
    Purpose
    Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes
    Insert
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
    Use
    Xenopus oocyte expression
    Tags
    Expression
    Mutation
    E55G, R237H, and K283R polymorphisms compared to …
    Promoter
    SP6
    Availability
    Academic Institutions and Nonprofits only
  3. pJM656

    Plasmid
    #53086
    Purpose
    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control
    Depositor
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Availability
    Academic Institutions and Nonprofits only
  4. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Insert
    LOV(wt)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. PA-DAD-dark

    Plasmid
    #53096
    Purpose
    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
    Insert
    LOV(dark)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus; C450M mutation in LOV2
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pSK-mPar1

    Plasmid
    #53227
    Purpose
    bacterial expression of mouse Par1
    Depositor
    Insert
    Par1 (F2r Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pBJ-mPar3

    Plasmid
    #53230
    Purpose
    mammalian expression of mouse Par3
    Depositor
    Insert
    Par3 (F2rl2 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-15 (native signal peptide)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pBJKS-mPar4

    Plasmid
    #53232
    Purpose
    mammalian expression of mouse Par4
    Depositor
    Insert
    Par4 (F2rl3 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-17 (native signal peptide)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 11277 results