12,479 results

1. pX330 p53

   Plasmid

   #59910

   Purpose

   pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting p53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSP64-mHCN2

   Plasmid

   #53060

   Purpose

   Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

   Insert

   Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   E55G, R237H, and K283R polymorphisms compared to …

   Promoter

   SP6

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE_GFP-GluR1

   Plasmid

   #34857

   Depositor

   Mark Mayford

   Article

   Matsuo et al Science. 2008 Feb 22;319(5866):1104-7.

   Insert

   GluR1 (Gria1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-2xFYVE

   Plasmid

   #140047

   Purpose

   Expresses GFP-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Talin1

   Plasmid

   #26724

   Depositor

   Anna Huttenlocher

   Article

   Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

   Insert

   Talin (Tln1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. Slc17a7-IRES2-Cre targeting vector

   Plasmid

   #61574

   Purpose

   Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Slc17a7-IRES2-Cre
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFIN-RK-GFP-mIRBP156-CHER-WPRE

   Plasmid

   #44351

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Inserts

   RK-GFP
   mIRBP156-CHER
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human rhodopsin kinase (pos 2554-2850) and mouse …

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCE-mp53DD

   Plasmid

   #41856

   Purpose

   Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   CAG

   Available since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

   Plasmid

   #194458

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-CD19 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Kir2.1-T2A-tdTomato

    Plasmid

    #60598

    Purpose

    Expresses Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

    Plasmid

    #194457

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-CD19 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid

    #79649

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid

    #79650

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    isoleucine 679 changed to lysine

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAD67 scFv [L127/8]

    Plasmid

    #190509

    Purpose

    Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)
    

    Use

    Tags

    Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-mSTING-EGFP

    Plasmid

    #214148

    Purpose

    Retroviral transduction of EGFP-tagged STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-sgR26

    Plasmid

    #127376

    Purpose

    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

    Plasmid

    #205442

    Purpose

    production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

    Depositor

    Jeremy Kay

    Article

    Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

    Insert

    Unc5c (Unc5c Mouse)
    

    Use

    AAV

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    mini-CMV (with MVM small intron)

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

    Plasmid

    #175549

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    WAPL (Wapl Mouse)
    

    Use

    Mouse Targeting

    Tags

    AID[71-114]-eGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge