Addgene

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pHL 1477

Plasmid

#69787

Purpose

KanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Insert

T7RNAP

Use

Synthetic Biology

Tags

OmpC leader

Expression

Bacterial

Mutation

Promoter

pTet

Availability

Academic Institutions and Nonprofits only
pHL 1701

Plasmid

#69788

Purpose

KanR with FRT sites + pT7::T710::CFP::T7 terminator::T1 terminator + ColE

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Insert

CFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pHL 2146

Plasmid

#69789

Purpose

KanR with FRT sites + pT7::SgrS::T1 terminator + ColE

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Insert

SgrS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pHL 2147

Plasmid

#69790

Purpose

KanR with FRT sites + pT7::LacO::SgrS::T1 terminator + ColE

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Insert

SgrS

Use

Synthetic Biology

Tags

LacO

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 VAMP2 R125TAG

Plasmid

#69876

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2-GFP (Vamp2 Rat)

Use

Synthetic Biology

Tags

GFP

Expression

Mammalian

Mutation

Amber stop codon in the linker between VAMP2 and …

Promoter

Availability

Academic Institutions and Nonprofits only
pN1 VAMP2 R125TAG

Plasmid

#69877

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2 (Vamp2 Rat)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Amber and Ochre stop codons in the former linker …

Promoter

Availability

Academic Institutions and Nonprofits only
circRAJ31

Plasmid

#69926

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PltetO1-2

Availability

Academic Institutions and Nonprofits only

Enlarge
cisRAJ31-GFP-LAA

Plasmid

#69928

Purpose

Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-GFP-LAA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PlLacO1

Availability

Academic Institutions and Nonprofits only

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circRAJ31-Toehold_mutant

Plasmid

#69930

Purpose

Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31_toeholdmutant

Use

Synthetic Biology

Tags

Expression

Mutation

4 bases mutated in toehold of riboregulator

Promoter

PltetO1-2

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7_circRAJ31_C873U

Plasmid

#69932

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31_C873U

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PlLacO1

Availability

Academic Institutions and Nonprofits only

Enlarge
taRAJ31min

Plasmid

#69934

Purpose

Expresses taRAJ31min, a linear riboregulator.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-taRAJ31min

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PltetO1-2

Availability

Academic Institutions and Nonprofits only

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Lasso 1

Plasmid

#69935

Purpose

The first of the two RNA lassos required for the RNA chain construction

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Availability

Academic Institutions and Nonprofits only

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Lasso 2

Plasmid

#69936

Purpose

The second of the two RNA lassos required for the RNA chain construction.

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Availability

Academic Institutions and Nonprofits only

Enlarge
AL562-2

Plasmid

#69937

Purpose

Construct targeting the PIE-target sequence.

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

AL562-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCKbreak12

Plasmid

#69938

Purpose

pSTC2,cisRaj12-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

CisRaj12-sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCKtheoRaj11

Plasmid

#69939

Purpose

pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj11,cisRaj11,sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCKtheoRaj12-olsa

Plasmid

#69940

Purpose

pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Inserts

TheoHHAzRaj12,cisRaj12,sfGFP
theoHHAzRaj12,cisRaj12-sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12-o11

Plasmid

#69941

Purpose

pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj12,cisRaj11,sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge