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3,554 results

Showing: 201 - 220 of 3554 results

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AByG

Plasmid

#111083

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCFD3-ovoD1

Plasmid

#111142

Purpose

Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

Depositor

Norbert Perrimon

Article

Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

Insert

pCFD3-ovoD1 (ovo Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG-FLAG PLU-HIS

Plasmid

#113173

Purpose

Baculovirus expression of PNG and PLU

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

PNG, PLU

Use

Baculovirus

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MEI-S332-GFP genomic in pCaSpeR

Plasmid

#113174

Purpose

In P element vector for transposon insertion into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-amo

Plasmid

#113190

Purpose

Expresses amo in vertebrate cells

Depositor

Craig Montell

Article

Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

Insert

amo (Pkd2 Fly)

Use

In vitro transcription

Tags

Expression

Mammalian

Mutation

Y602H

Promoter

simian CMV IE94

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati Exons 1-3

Plasmid

#110113

Purpose

Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS Exon

Plasmid

#110119

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Flag-Hel25E MUT

Plasmid

#110122

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

4 mutations (KKLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E WT

Plasmid

#110123

Purpose

Expresses Flag-tagged D. melanogaster Hel25E

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3. 3xFlag:PP4-19c[D85NH115N]

Plasmid

#112247

Purpose

expression of fly mutant PP4

Depositor

Simone Di Giovanni

Insert

PP4 (Pp4-19C Fly)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D85NH115N

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
20XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111544

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson_tdtomato_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge
5XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111545

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChrimson_tdtomato_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge
20XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111546

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge
10XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111547

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge
13XLexAop2 IVS ChrimsonR mCherry_tr

Plasmid

#111548

Purpose

Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge
20XUAS IVS Jaws mCherry_tr

Plasmid

#111549

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge
10XUAS IVS Jaws mCherry_tr

Plasmid

#111550

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only

Enlarge