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TALEngineering icon

Joung Lab REAL Assembly TALEN kit
(Kit # 1000000017 )

This 32 plasmid TALEN kit is used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods described by the Joung lab.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$350 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sanders JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung KJ, Yeh JRJ. Nat Biotechnol. 2011 Aug 5;29(8):697-8. PubMed

Description

The Joung Lab has described a rapid and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol. 2011) as well as REAL-Fast and FLASH methods. The Joung lab TALEN kit utilizes a set of 28 plasmids each encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays and 4 different TALEN expression vectors. DNA fragments encoding TALE repeat arrays can be easily transferred by simple cloning into one of four different TALEN expression vectors (each possessing one of the four different carboxy-terminal 0.5 TALE repeat domains). Using REAL, REAL-Fast or FLASH assemnbly methods, any combination of TALE repeats can be assembled into arrays of any desired length.

Kit Documentation

Icon Joung lab TALEN kit.pdf (368.2 KB)

Resources

More detailed information about TALE assembly, including detailed and up-to-date protocols, can be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup

An upgraded version of the ZiFiT Targeter program is available to enable users to identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. The software is publicly available without registration at: http://zifit.partners.org.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017)"

For your Reference section:
Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sanders JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung KJ, Yeh JRJ. Nat Biotechnol. 2011 Aug 5;29(8):697-8. PubMed

Resistance Color Key:

 
  Ampicillin  

Inventory

Well Plasmid Resistance
A / 1 TAL006
  Ampicillin
A / 2 TAL007
  Ampicillin
A / 3 TAL009
  Ampicillin
A / 4 TAL010
  Ampicillin
A / 5 TAL011
  Ampicillin
A / 6 TAL012
  Ampicillin
A / 7 TAL014
  Ampicillin
A / 8 TAL015
  Ampicillin
A / 9 TAL016
  Ampicillin
A / 10 TAL017
  Ampicillin
A / 11 TAL019
  Ampicillin
A / 12 TAL020
  Ampicillin
B / 1 TAL021
  Ampicillin
B / 2 TAL022
  Ampicillin
B / 3 TAL024
  Ampicillin
B / 4 TAL025
  Ampicillin
B / 5 TAL026
  Ampicillin
B / 6 TAL027
  Ampicillin
B / 7 TAL029
  Ampicillin
B / 8 TAL030
  Ampicillin
B / 9 TAL031
  Ampicillin
B / 10 TAL032
  Ampicillin
B / 11 TAL034
  Ampicillin
B / 12 TAL035
  Ampicillin
C / 1 TAL036
  Ampicillin
C / 2 TAL037
  Ampicillin
C / 3 TAL039
  Ampicillin
C / 4 TAL040
  Ampicillin
C / 5 JDS70
  Ampicillin
C / 6 JDS71
  Ampicillin
C / 7 JDS74
  Ampicillin
C / 8 JDS78
  Ampicillin
Data from: CALCULATED @ 2016-07-28 10:37:49.423946
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