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Zinc Finger Consortium: Nuclease Expression Vectors
This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique cloning sites.
Vectors encoding wild-type FokI nuclease domains:
Vectors encoding heterodimeric FokI nuclease domains:
- pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et al., Nat Biotech 2007) in mammalian cells or zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009)
- pMLM292 (for expression of a “-” heterodimeric FokI domain. Miller et al., Nat Biotech 2007) in mammalian cells or zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009)
- pMLM800 (for expression of a ZFN targeted to a site with a spacer of 7 bps and harboring a “+” heterodimeric FokI domain (Miller et al., Nat Biotech 2007) in mammalian cells or zebrafish).
- pMLM802 (for expression of a ZFN targeted to a site with a spacer of 7 bps and harboring a “-” heterodimeric FokI domain (Miller et al., Nat Biotech 2007) in mammalian cells or zebrafish).
- pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-” heterodimeric FokI domain (Sollu et al., Nucleic Acids Res) in mammalian cells or zebrafish).
- pCLR2068 (for expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “+” KKR heterodimeric FokI domain. (Doyon et al., Nat Methods 2011) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al., Nucleic Acids Res 2012)
- pCLR2070 (for expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “-” ELD heterodimeric FokI domain. (Doyon et al., Nat Methods 2011) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al., Nucleic Acids Res 2012)