zfclogo.jpgZinc Finger Consortium: Nuclease Expression Vectors

This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique cloning sites.

Vectors encoding wild-type FokI nuclease domains:

  • pST1374 (for expression in mammalian cells or zebrafish)
  • pDW1775 (for expression in plants)

Vectors encoding heterodimeric FokI nuclease domains: