PurposeExpresses BE4 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100802||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3402
- Total vector size (bp) 8877
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesR. norvegicus (rat), Synthetic; S. pyogenes
Insert Size (bp)5475
- Promoter CMV
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BE4 was a gift from David Liu (Addgene plasmid # 100802 ; http://n2t.net/addgene:100802 ; RRID:Addgene_100802)
For your References section:Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. aao4774 [pii] PubMed 28875174