PurposeThis is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101053||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2941
- Total vector size (bp) 9120
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameCryptococcus mCherry expression plasmid
- Promoter Cryptococcus Histone3 promoter
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CGTTGTTTCAGGCCTGCGGATG
- 3′ sequencing primer CCTTCATCAACATTCTGACG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bymCherry gene is cloned from plasmid pLK25 a gift from Dr. Peter Williamson from NIH.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pH3mCHSH2 was a gift from Jennifer Lodge (Addgene plasmid # 101053 ; http://n2t.net/addgene:101053 ; RRID:Addgene_101053)
For your References section:A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome. Upadhya R, Lam WC, Maybruck B, Donlin MJ, Chang AL, Kayode S, Ormerod KL, Fraser JA, Doering TL, Lodge JK. Fungal Genet Biol. 2017 Sep 1. pii: S1087-1845(17)30136-6. doi: 10.1016/j.fgb.2017.08.008. 10.1016/j.fgb.2017.08.008 PubMed 28870457