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pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
(Plasmid #101108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101108 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 101108-D50 50 μg of DNA in Tris buffer $495
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DNA 101108-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTMt_NES_TCS(Q’G)_HA-dCas9( N)_P2A-Puro-WPRE
  • Modifications to backbone
    The TMt, NES and TCS(Q’G) sequences from pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE were removed and replaced with the membrane localisation signal/FLAG tag/BDKBR2 coding sequence/V2 tail from plasmid BDKBR2-Tango and the TEV cleavage site ENLYFQL.
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BDKRB2-dCas9(N)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    BDKBR2-Tango (gift from Bryan Roth, Addgene plasmid #66230); pTMt_NES_TCS(Q’G)_HAdCas9(N)_P2A-Puro-WPRE.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE was a gift from Tudor Fulga (Addgene plasmid # 101108 ; http://n2t.net/addgene:101108 ; RRID:Addgene_101108)

  • For your References section:

    Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Baeumler TA, Ahmed AA, Fulga TA. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. 10.1016/j.celrep.2017.08.044 PubMed 28903044
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