PurposeEncodes the fluorescent protein EG-4 for constitutive expression in E. coli.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101640||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)630
/ Fusion Protein
- 6XHis (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Created in the lab of Nathan Shaner at the Scintillon Institute.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSM15 was a gift from Thomas Hughes (Addgene plasmid # 101640 ; http://n2t.net/addgene:101640 ; RRID:Addgene_101640)
For your References section:Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation. Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, Hughes TE, Drobizhev M. J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25. 10.1021/acs.jpclett.7b00960 PubMed 28530831