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pCXN2-FLAG-AT2R-CFP
(Plasmid #101660)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101660 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCXN2
  • Total vector size (bp) 7840
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha is also a suitable growth strain.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Angiotensin II type 2 receptor
  • Alt name
    AT2R
  • Alt name
    AGTR2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1124
  • GenBank ID
    NM_000686.4
  • Entrez Gene
    AGTR2 (a.k.a. AT2, ATGR2, MRX88)
  • Promoter CAG promoter
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (with FLAG tag) (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCXN2-FLAG-AT2R-CFP was a gift from Yusuke Ohba (Addgene plasmid # 101660 ; http://n2t.net/addgene:101660 ; RRID:Addgene_101660)
  • For your References section:

    Attenuation of ligand-induced activation of angiotensin II type 1 receptor signaling by the type 2 receptor via protein kinase C. Inuzuka T, Fujioka Y, Tsuda M, Fujioka M, Satoh AO, Horiuchi K, Nishide S, Nanbo A, Tanaka S, Ohba Y. Sci Rep. 2016 Feb 9;6:21613. doi: 10.1038/srep21613. 10.1038/srep21613 PubMed 26857745