pTriEX-Antenna-GDI Cdc42
(Plasmid
#101681)
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Purpose"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 101681-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 101681-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTrieEX
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Backbone manufacturerEMD Millipore
- Backbone size w/o insert (bp) 5238
- Total vector size (bp) 7038
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc42
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Alt nameG25K GTP-binding protein
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SpeciesH. sapiens (human)
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Insert Size (bp)2068
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GenBank IDAAA52592.1
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Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
- Promoter CMV
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- FRET Antenna (mCerulean-cpVenus) (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pTriEx up
- 3′ sequencing primer pTriEx down
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 101681-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 101681-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTriEX-Antenna-GDI Cdc42 was a gift from Klaus Hahn (Addgene plasmid # 101681 ; http://n2t.net/addgene:101681 ; RRID:Addgene_101681) -
For your References section:
FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Hodgson L, Spiering D, Sabouri-Ghomi M, Dagliyan O, DerMardirossian C, Danuser G, Hahn KM. Nat Chem Biol. 2016 Aug 8. doi: 10.1038/nchembio.2145. 10.1038/nchembio.2145 PubMed 27501396
