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pET28a-ybbr-HIS-ddFLN4-K10
(Plasmid #101718)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101718 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5216
  • Total vector size (bp) 5651
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ddFLN4 fingerprint, K10 peptide
  • Species
    H. sapiens (human); Dictyostelium discoideum
  • Insert Size (bp)
    435
  • Mutation
    C18S in ddFLN4
  • Promoter T7
  • Tags / Fusion Proteins
    • HIS (N terminal on insert)
    • ybbr (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer CTAGTTATTGCTCAGCGGT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-ybbr-HIS-ddFLN4-K10 was a gift from Hermann Gaub (Addgene plasmid # 101718)
  • For your References section:

    Molecular mechanism of extreme mechanostability in a pathogen adhesin. Milles LF, Schulten K, Gaub HE, Bernardi RC,. Science 30 Mar 2018: Vol. 359, Issue 6383, pp. 1527-1533 10.1126/science.aar2094