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pLenti TurboRFP BlastR
(Plasmid #102343)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102343 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCIG3 #78264
  • Backbone manufacturer
    Goodrum Lab, University of Arizona
  • Backbone size w/o insert (bp) 7353
  • Total vector size (bp) 8046
  • Modifications to backbone
    Subcloned in Blasticidin resistance gene in place of GFP behind the IRES sequence
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TurboRFP
  • Species
    Synthetic
  • Insert Size (bp)
    693
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti TurboRFP BlastR was a gift from Ghassan Mouneimne (Addgene plasmid # 102343 ; http://n2t.net/addgene:102343 ; RRID:Addgene_102343)
  • For your References section:

    High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons. Parker SS, Moutal A, Cai S, Chandrasekaran S, Roman MR, Koshy AA, Khanna R, Zinsmaier KE, Mouneimne G. eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-0135-18. doi: 10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct. eN-MNT-0135-18 [pii] PubMed 30263951