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pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV
(Plasmid #102422)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102422 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPB-hCMV1-hNANOG-IV
  • Backbone manufacturer
    Austin Smith (Addgene plasmid# 60432)
  • Modifications to backbone
    human NANOG removed to insert Tet1 (201R2)
  • Vector type
    Mammalian Expression
  • Selectable markers
    mVenus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet1 catalytic domain mutant
  • Species
    M. musculus (mouse)
  • Mutation
    H1620Y and D1622A mutations in Tet1 catalytic domain; Modified at the Dharmacon SMARTpool siRNA #2 target site to make it siRNA-resistant
  • GenBank ID
    NM_027384.1
  • Entrez Gene
    Tet1 (a.k.a. 2510010B09Rik, AA517754, BB001228, Cxxc6, D10Ertd17, D10Ertd17e, LCX, mKIAA1676)
  • Promoter TetO-CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site blunt end (destroyed during cloning)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Depositor Comments

The full length transcript 201 refers to the coding sequence (CDS) of Ensembl transcript Tet1-201 (transcript ID ENSMUST00000050826.13) or NCBI RefSeq NM_027384.1. This variant (isoform 2) lacks an alternate in-frame exon in the central coding region, compared to variant 1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV was a gift from Kian Peng Koh (Addgene plasmid # 102422 ; http://n2t.net/addgene:102422 ; RRID:Addgene_102422)
  • For your References section:

    Lineage-specific functions of TET1 in the postimplantation mouse embryo. Khoueiry R, Sohni A, Thienpont B, Luo X, Velde JV, Bartoccetti M, Boeckx B, Zwijsen A, Rao A, Lambrechts D, Koh KP. Nat Genet. 2017 Jul;49(7):1061-1072. doi: 10.1038/ng.3868. Epub 2017 May 15. 10.1038/ng.3868 PubMed 28504700