pSG75-β2AR-T4L-sfGFP
(Plasmid #102461)

• Purpose: Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
• Depositing Lab: Richard Murray
• Publication: Guo et al bioRxiv 104455

Backbone

• Vector backbone: pBEST
• Backbone size w/o insert (bp): 2525
• Total vector size (bp): 4793
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): KL740
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: β2AR
• Alt name: β2 adrenergic receptor/T4 lysozyme chimera
• Alt name: 2RH1
• Species: H. sapiens (human)
• Insert Size (bp): 1479
• Promoter: OR2-OR1-Pr
• Tags / Fusion Proteins:
  • sfGFP (C terminal on insert)
  • His tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AAAACCGAATTTTGCTGGGTG
• 3′ sequencing primer: ATGATAAAGAACACAGTCATAAGTGC

Resource Information

• Supplemental Documents:
  • pSG75_ b2AR-T4L plasmid.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/01/30/104455 for bioRxiv preprint.