Expressing Biologically Active Membrane Proteins in a Cell-Free Transcription-Translation Platform
Guo S, Vaish A, Chen Q, Murray RM
bioRxiv 104455 Article
Guo S, Vaish A, Chen Q, Murray RM
bioRxiv 104455 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102457 | pSG66-KcsA-Kv1.3-sfGFP | Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter. |
| 102458 | pSG69-KcsA-Kv1.3-deGFP | Expresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site. |
| 102459 | pSG73-β1ARts-sfGFP | Expresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter. |
| 102460 | pSG74-β2AR-sfGFP | Expresses a fusion protein of wild type β2 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter. |
| 102461 | pSG75-β2AR-T4L-sfGFP | Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter. |