pRSF-FGE
(Plasmid
#102615)
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PurposeInducible expression of Formylglycine-generating enzyme
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSF-Duet
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFormylglycine-generating enzyme
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Alt nameNP_215226
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Alt nameRv0712
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PfuI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FSG gene on PfoI-XhoI fragment from pBAD/myc-hisA Rv0712 (FGE) (Carolyn Bertozzi, Addgene plasmid # 16132) was cloned into pRSF-Duet PfoI-XhoI cut
FGE(rv0712) is nt 4810-5709
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF-FGE was a gift from Marc Wold (Addgene plasmid # 102615 ; http://n2t.net/addgene:102615 ; RRID:Addgene_102615) -
For your References section:
Single-Molecule Analysis of Replication Protein A-DNA Interactions. Bain FE, Fischer LA, Chen R, Wold MS. Methods Enzymol. 2018;600:439-461. doi: 10.1016/bs.mie.2017.11.016. 10.1016/bs.mie.2017.11.016 PubMed 29458769
Map uploaded by the depositor.
