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Addgene

Ad-EFNA1
(Plasmid #102724)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102724 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAd Easy1
  • Vector type
    Adenoviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EFNA1
  • Alt name
    EphrinA1
  • Alt name
    B61
  • Species
    H. sapiens (human)
  • Entrez Gene
    EFNA1 (a.k.a. B61, ECKLG, EFL1, EPLG1, GMAN, LERK-1, LERK1, TNFAIP4)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct is a adenoviral vector which carries human EFNA1 cDNA and ready to produce adenovirus which express Ephrin-A1 by tranfection into 293A cells followed by PacI digestion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ad-EFNA1 was a gift from Jin Chen (Addgene plasmid # 102724 ; http://n2t.net/addgene:102724 ; RRID:Addgene_102724)
  • For your References section:

    Ephrin-A1 facilitates mammary tumor metastasis through an angiogenesis-dependent mechanism mediated by EphA receptor and vascular endothelial growth factor in mice. Brantley-Sieders DM, Fang WB, Hwang Y, Hicks D, Chen J. Cancer Res. 2006 Nov 1;66(21):10315-24. 10.1158/0008-5472.CAN-06-1560 PubMed 17079451