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Y771F EphA2 pcDNA3
(Plasmid #102735)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102735 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EphA2
  • Alt name
    Eck
  • Species
    M. musculus (mouse)
  • Mutation
    Y771F (TAC > TTC)
  • Entrez Gene
    Epha2 (a.k.a. AW545284, Eck, Myk2, Sek-2, Sek2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Y771F EphA2 pcDNA3 was a gift from Jin Chen (Addgene plasmid # 102735 ; http://n2t.net/addgene:102735 ; RRID:Addgene_102735)
  • For your References section:

    Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase. Fang WB, Brantley-Sieders DM, Hwang Y, Ham AJ, Chen J. J Biol Chem. 2008 Jun 6;283(23):16017-26. doi: 10.1074/jbc.M709934200. Epub 2008 Apr 3. 10.1074/jbc.M709934200 PubMed 18387945