STAGR_SAMScaffold_hU6
(Plasmid
#102843)
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PurposeCan be used as PCR template for a STAgR reaction
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneHuman gRNA Expression Vector / PCR Template for STAgR
- Backbone size w/o insert (bp) 3250
- Total vector size (bp) 3668
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Vector typeas PCR template
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegRNAScaffold_hU6promoter
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Insert Size (bp)380
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTTTTAGAGCTAGAAATAGCAAGTT
- 3′ sequencing primer CGGTGTTTCGTCCTTT (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STAGR_SAMScaffold_hU6 was a gift from Stefan Stricker (Addgene plasmid # 102843 ; http://n2t.net/addgene:102843 ; RRID:Addgene_102843) -
For your References section:
One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR). Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A, Gotz M, Ninkovic J, Stricker SH. PLoS One. 2018 Apr 27;13(4):e0196015. doi: 10.1371/journal.pone.0196015. eCollection 2018. 10.1371/journal.pone.0196015 PubMed 29702666
