One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).
Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A, Gotz M, Ninkovic J, Stricker SH
PLoS One. 2018 Apr 27;13(4):e0196015. doi: 10.1371/journal.pone.0196015. eCollection 2018. PubMed Article
Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A, Gotz M, Ninkovic J, Stricker SH
PLoS One. 2018 Apr 27;13(4):e0196015. doi: 10.1371/journal.pone.0196015. eCollection 2018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102840 | STAGR_gRNAScaffold_hU6 | Can be used as PCR template for a STAgR reaction |
| 102841 | STAGR_gRNAScaffold_hH1 | Can be used as PCR template for a STAgR reaction |
| 102842 | STAGR_gRNAScaffold_h7SK | Can be used as PCR template for a STAgR reaction |
| 102843 | STAGR_SAMScaffold_hU6 | Can be used as PCR template for a STAgR reaction |
| 102844 | STAGR_gRNAScaffold_mU6 | Can be used as PCR template for a STAgR reaction |
| 102845 | STAGR_SAMScaffold_mU6 | Can be used as PCR template for a STAgR reaction |
| 102846 | STAGR_SAMScaffold_h7SK | Can be used as PCR template for a STAgR reaction |
| 102847 | STAGR_SAMScaffold_hH1 | Can be used as PCR template for a STAgR reaction |
| 102992 | STAgR_Neo | Can be used as backbone for a STAgR reaction |
| 102993 | STAgR_tdTomato | Can be used as backbone for a STAgR reaction |