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One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).
Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A, Gotz M, Ninkovic J, Stricker SH
PLoS One. 2018 Apr 27;13(4):e0196015. doi: 10.1371/journal.pone.0196015. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
102840STAGR_gRNAScaffold_hU6Can be used as PCR template for a STAgR reaction
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102841STAGR_gRNAScaffold_hH1Can be used as PCR template for a STAgR reaction
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102842STAGR_gRNAScaffold_h7SKCan be used as PCR template for a STAgR reaction
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102843STAGR_SAMScaffold_hU6Can be used as PCR template for a STAgR reaction
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102844STAGR_gRNAScaffold_mU6Can be used as PCR template for a STAgR reaction
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102845STAGR_SAMScaffold_mU6Can be used as PCR template for a STAgR reaction
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102846STAGR_SAMScaffold_h7SKCan be used as PCR template for a STAgR reaction
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102847STAGR_SAMScaffold_hH1Can be used as PCR template for a STAgR reaction
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102992STAgR_NeoCan be used as backbone for a STAgR reaction
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102993STAgR_tdTomatoCan be used as backbone for a STAgR reaction
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