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PurposeOverexpresses KIF1A and cell fill TdTomato
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 102850-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 102850-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepeGFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 9902
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Modifications to backboneEGFP replaced by tdTomato
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF1A
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Alt nameATSV, Kns1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)5085
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Entrez GeneKif1a (a.k.a. A, ATSV, C630002N23Rik, Gm1626, Kn, Kns1)
- Promoter CMV
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Tag
/ Fusion Protein
- tdTomato (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI or AgeI (unknown if destroyed)
- 3′ cloning site ScaI (unknown if destroyed)
- 5′ sequencing primer tdTomato-Fwd
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The rat eGFP-KIF1A was a kind gift from Gary Banker (OHSU).
The tdTomato-KIF1A plasmid was constructed by restriction site removal of the eGFP from the eGFP-KIF1A plasmid and insertion of tdTomato.
Addgene NGS results found S33N within the KIF1A translation compared to NCBI reference sequence XP_017459420.1
DNA (Catalog # 102850-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 102850-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tdTomato-KIF1A was a gift from Erik Dent (Addgene plasmid # 102850 ; http://n2t.net/addgene:102850 ; RRID:Addgene_102850) -
For your References section:
Transport of a kinesin-cargo pair along microtubules into dendritic spines undergoing synaptic plasticity. McVicker DP, Awe AM, Richters KE, Wilson RL, Cowdrey DA, Hu X, Chapman ER, Dent EW. Nat Commun. 2016 Sep 23;7:12741. doi: 10.1038/ncomms12741. 10.1038/ncomms12741 PubMed 27658622
