PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102908||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-EEA-g1-PGK-Puro was a gift from Timo Otonkoski (Addgene plasmid # 102908 ; http://n2t.net/addgene:102908 ; RRID:Addgene_102908)
For your References section:Human pluripotent reprogramming with CRISPR activators. Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti EM, Trokovic R, Kere J, Otonkoski T. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. 10.1038/s41467-018-05067-x PubMed 29980666