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pLVH-EF1a-GFP-P2A-KLF4
(Plasmid #130694)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 130694 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVH-EF1a-GFP-P2A
  • Backbone manufacturer
    Hu Lab
  • Backbone size w/o insert (bp) 8277
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human KLF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1413
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Resource Information

Depositor Comments

We extensively modified the plasmid of pLVX-AcGFP-C1 (Clontech, 632155) by replacing CMV promoter with EF1a promoter, adding P2A to mediate co-expression of the gene of interest, and removal of PGK promoter and puromycin resistant gene to make a lentiviral backbone vector of pLVH-EF1a-P2A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVH-EF1a-GFP-P2A-KLF4 was a gift from Kejin Hu (Addgene plasmid # 130694 ; http://n2t.net/addgene:130694 ; RRID:Addgene_130694)
  • For your References section:

    The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok MA, Crossman DK, Townes TM, Hu K. Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869. 10.1038/ncomms10869 PubMed 26947130