pCAG-GBP-dCas9-mRFP
(Plasmid
#102933)
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PurposeExpression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)
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Depositing Lab
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Depositing OrganizationLudwig-Maximilians-Universitaet Muenchen (LMU)
Located in Germany -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 102933-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 102933-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- GBP (GFP binding protein) (N terminal on insert)
- mRFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The GBP (GFP binding protein) and mRFP coding sequences were amplified from pGFPbinderImR (PMID 18622019) and cloned into pCAG-dCas9 (PMID 24637835) via XbaI and AsiSI/NotI, respectively.
DNA (Catalog # 102933-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 102933-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-GBP-dCas9-mRFP was a gift from Sebastian Bultmann (Addgene plasmid # 102933 ; http://n2t.net/addgene:102933 ; RRID:Addgene_102933) -
For your References section:
Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system. Anton T, Bultmann S. Nucleus. 2017 May 4;8(3):279-286. doi: 10.1080/19491034.2017.1292194. Epub 2017 Feb 23. 10.1080/19491034.2017.1292194 PubMed 28448738