PurposePlasmid encoding mYPet
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||103988||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3674
- Total vector size (bp) 4391
Modifications to backboneDerived from pUA66. Inserted constitutive promoter proC. Replaced original insert with FP of interest.
Vector typeBacterial Expression ; Low copy
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)717
MutationMutations relative to wild-type GFP (F46L, I47L, F64L, S65G, S72A, M153T, V163A, S175G, T203Y, A206K, S208F, V224L, H231E, D234N)
- Promoter proC
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGTATCACGAGGCCCTTTC
- 3′ sequencing primer AAAGGGAAAACTGTCCATATGCAC (Common Sequencing Primers)
This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEB1-mYPet was a gift from Philippe Cluzel (Addgene plasmid # 103988 ; http://n2t.net/addgene:103988 ; RRID:Addgene_103988)
For your References section:Systematic characterization of maturation time of fluorescent proteins in living cells. Balleza E, Kim JM, Cluzel P. Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 2017 Nov 20. 10.1038/nmeth.4509 PubMed 29320486