Cherry-HA-KCC2
(Plasmid
#104077)
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PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
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Depositing Lab
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Depositing OrganizationINSERM U901, INSTITUT DE NEUROBIOLOGIE DE LA MEDITERRANEE (INMED)
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 104077-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 104077-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmCherry-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4731
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Modifications to backboneCMV promoter replaced by UbC promoter
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKCC2 (b isoform)
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Alt nameSlc12a5
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3350
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MutationEcoR1 site in rat KCC2 was removed by silent mutation, A3267G in rat coding sequence
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Entrez GeneSlc12a5 (a.k.a. Kcc2)
- Promoter UbC
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Tag
/ Fusion Protein
- mCherry-HA tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site Eco R1 (unknown if destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 104077-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 104077-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cherry-HA-KCC2 was a gift from Igor Medyna (Addgene plasmid # 104077 ; http://n2t.net/addgene:104077 ; RRID:Addgene_104077) -
For your References section:
Genetically encoded impairment of neuronal KCC2 cotransporter function in human idiopathic generalized epilepsy. Kahle KT, Merner ND, Friedel P, Silayeva L, Liang B, Khanna A, Shang Y, Lachance-Touchette P, Bourassa C, Levert A, Dion PA, Walcott B, Spiegelman D, Dionne-Laporte A, Hodgkinson A, Awadalla P, Nikbakht H, Majewski J, Cossette P, Deeb TZ, Moss SJ, Medina I, Rouleau GA. EMBO Rep. 2014 Jul;15(7):766-74. doi: 10.15252/embr.201438840. Epub 2014 Jun 13. 10.15252/embr.201438840 PubMed 24928908
Map uploaded by the depositor.
