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(Plasmid #61404)


Item Catalog # Description Quantity Price (USD)
Plasmid 61404 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6900
  • Total vector size (bp) 10292
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
    AAG43493.1 AF208159.1
  • Entrez Gene
    SLC12A5 (a.k.a. EIEE34, EIG14, KCC2, hKCC2)
  • Promoter CAG
  • Tag / Fusion Protein
    • IRES-tdTomato-Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer CTACAGCTCCTGGGCAACGT
  • 3′ sequencing primer CGGCTTCGGCCAGTAACG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCITF-KCC2 was a gift from Franck Polleux (Addgene plasmid # 61404 ; ; RRID:Addgene_61404)
  • For your References section:

    KCC2 expression promotes the termination of cortical interneuron migration in a voltage-sensitive calcium-dependent manner. Bortone D, Polleux F. Neuron. 2009 Apr 16;62(1):53-71. doi: 10.1016/j.neuron.2009.01.034. 10.1016/j.neuron.2009.01.034 PubMed 19376067