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pCB213 (pHUsp2-cc)
(Plasmid #104338)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104338 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Usp2-cc
  • Alt name
    AAV27298.1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1137
  • Mutation
    catalytic core of mouse Usp2
  • Entrez Gene
    Usp2 (a.k.a. MNCb-0190, B930035K21Rik, Ubp41)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB213 (pHUsp2-cc) was a gift from Rohan Baker & Alexander Varshavsky (Addgene plasmid # 104338)

  • For your References section:

    An efficient system for high-level expression and easy purification of authentic recombinant proteins. Catanzariti AM, Soboleva TA, Jans DA, Board PG, Baker RT. Protein Sci. 2004 May;13(5):1331-9. 10.1110/ps.04618904 PubMed 15096636
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