PurposeAAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104356||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
- Promoter smCBA
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2143 was a gift from Elizabeth Simpson (Addgene plasmid # 104356 ; http://n2t.net/addgene:104356 ; RRID:Addgene_104356)
For your References section:rAAV-compatible MiniPromoters for restricted expression in the brain and eye. de Leeuw CN, Korecki AJ, Berry GE, Hickmott JW, Lam SL, Lengyell TC, Bonaguro RJ, Borretta LJ, Chopra V, Chou AY, D'Souza CA, Kaspieva O, Laprise S, McInerny SC, Portales-Casamar E, Swanson-Newman MI, Wong K, Yang GS, Zhou M, Jones SJ, Holt RA, Asokan A, Goldowitz D, Wasserman WW, Simpson EM. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. 10.1186/s13041-016-0232-4 [pii] PubMed 27164903