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Purpose(Empty Backbone) A piggybac vector with a U6 promoter that allows cloning of guide RNAs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104536 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePiggybac
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Backbone manufacturerSystems Biosciences
- Backbone size (bp) 4700
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Vector typeMammalian Expression, Mouse Targeting, CRISPR, Synthetic Biology ; Piggybac transposon
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-U6insert was a gift from George Church (Addgene plasmid # 104536 ; http://n2t.net/addgene:104536 ; RRID:Addgene_104536) -
For your References section:
Developmental barcoding of whole mouse via homing CRISPR. Kalhor R, Kalhor K, Mejia L, Leeper K, Graveline A, Mali P, Church GM. Science. 2018 Aug 9. pii: science.aat9804. doi: 10.1126/science.aat9804. 10.1126/science.aat9804 PubMed 30093604
