pENTR-li-p33_RS-CLIP
(Plasmid #104634)

• Purpose: Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)
• Depositing Lab: Sébastien Walchli
• Publication: Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663. doi: 10.1080/2162402X.2018.1558663. eCollection 2019.

Backbone

• Vector backbone: pENTR
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2580
• Total vector size (bp): 3510
• Vector type: entry vector for Gateway system

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: modified Invariant chain p33
• Alt name: Ii
• Alt name: CD74
• Species: H. sapiens (human)
• Insert Size (bp): 602
• Mutation: CLIP domain was replaced by 2 restriction sites
• GenBank ID: CD74 molecule (Homo sapiens) NM_001025159.2
• Entrez Gene: CD74 (a.k.a. CLIP, DHLAG, HLADG, II, Ia-GAMMA, p33)
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: CAG GAA ACA GCT ATG ACC
• 3′ sequencing primer: TGT AAA ACG ACG GCC AGT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Tone F. Gregers, received through a collaboration

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original gene comes from Dr. Tone Gregers previous construct, we subcloned it into pENTR and performed the modification of the CLIP domain