pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
(Plasmid #105643)

• Purpose: Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis
• Depositing Lab: Sandra Rieger
• Publication: Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):E2189-98. doi: 10.1073/pnas.1525096113. Epub 2016 Mar 28.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone manufacturer: Chi-Bin Chien lab
• Backbone size w/o insert (bp): 5881
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
• Species: D. rerio (zebrafish)
• Insert Size (bp): 6800

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry