MT2a
(Plasmid
#105693)
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PurposeBacterial expression plasmids for production of recombinant metallothionein 2a
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTyb21
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMetallothionein 2a
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SpeciesH. sapiens (human)
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Insert Size (bp)183
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Entrez GeneMT2A (a.k.a. MT-2, MT-II, MT2)
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Tag
/ Fusion Protein
- Chiting Binding Domanin-Intein (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SapI (destroyed during cloning)
- 3′ cloning site PstI (not destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MT2a was a gift from Artur Krężel (Addgene plasmid # 105693 ; http://n2t.net/addgene:105693 ; RRID:Addgene_105693) -
For your References section:
Crosstalk of the structural and zinc buffering properties of mammalian metallothionein-2. Drozd A, Wojewska D, Peris-Diaz MD, Jakimowicz P, Krezel A. Metallomics. 2018 Apr 25;10(4):595-613. doi: 10.1039/C7MT00332C. 10.1039/C7MT00332C PubMed 29561927
