pET11a asyn Q99E (TAC-->TAT)
PurposeBacterial expression of mutant human alpha synuclein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105734||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5677
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namealpha-synuclein Q99E (TAC-->TAT)
SpeciesH. sapiens (human)
Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter T7
- Cloning method Unknown
- 5′ sequencing primer T7 (TAATACGACTCACTATAGG)
- 3′ sequencing primer T7 term (CCGCTGAGCAATAACTAGC) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET11a asyn Q99E (TAC-->TAT) was a gift from Hilal Lashuel (Addgene plasmid # 105734 ; http://n2t.net/addgene:105734 ; RRID:Addgene_105734)