pMB-SaCas9
(Plasmid #105998)

• Purpose: SaCas9 expression vector
• Depositing Lab: Mike McManus
• Publication: Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062.

Backbone

• Vector backbone: pBR322
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Hygromycin ; mouse CD4

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cas9 nuclease
• Alt name: SauCas9
• Species: Staphylococcus aureus
• Insert Size (bp): 3343
• Mutation: none
• Promoter: short EF1alpha
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ggcgcggggtaaactggg

Resource Information

• Supplemental Documents:
  • Extended Data 3 - SaCas9 nuclease vector.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results show a C-terminal HA tag rather than 3x HA tag. Also, the mCD4 selection marker matches NCBI reference NP_038516.1 and contains residues 1-427. Plasmids should function as described in the associated publication.