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Lyn-iTrkA-mCh-CRY2
(Plasmid #106169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106169 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 106169-D50 50 μg of DNA in Tris buffer $495
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DNA 106169-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmCherry N1
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lyn iTrkA mCherry CRY2PHR
  • Alt name
    CRY2
  • Alt name
    TrkA
  • Species
    R. norvegicus (rat), A. thaliana (mustard weed)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lyn-iTrkA-mCh-CRY2 was a gift from Bianxiao Cui (Addgene plasmid # 106169 ; http://n2t.net/addgene:106169 ; RRID:Addgene_106169)

  • For your References section:

    Optical Activation of TrkA Signaling. Duan L, Hope JM, Guo S, Ong Q, Francois A, Kaplan L, Scherrer G, Cui B. ACS Synth Biol. 2018 Jul 20;7(7):1685-1693. doi: 10.1021/acssynbio.8b00126. Epub 2018 Jul 12. 10.1021/acssynbio.8b00126 PubMed 29975841
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