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pAV-U6+27-tCorn
(Plasmid #106233)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106233 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAV U6+27
  • Backbone manufacturer
    Addgene plasmid # 25709
  • Backbone size w/o insert (bp) 6092
  • Total vector size (bp) 6252
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tRNA-Corn
  • Alt name
    tCorn
  • Species
    Synthetic
  • Insert Size (bp)
    160
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer TGACACACATTCCACAGCC
  • 3′ sequencing primer CGGCCGCCATATGGATATC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV-U6+27-tCorn was a gift from Samie Jaffrey (Addgene plasmid # 106233 ; http://n2t.net/addgene:106233 ; RRID:Addgene_106233)
  • For your References section:

    Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Song W, Filonov GS, Kim H, Hirsch M, Li X, Moon JD, Jaffrey SR. Nat Chem Biol. 2017 Nov;13(11):1187-1194. doi: 10.1038/nchembio.2477. Epub 2017 Sep 25. 10.1038/nchembio.2477 PubMed 28945233