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pAV5S-F30-2xdBroccoli
(Plasmid #66845)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66845 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAV-5S
  • Backbone size w/o insert (bp) 6064
  • Total vector size (bp) 6298
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Two dimeric Broccoli aptamers in the F30 scaffold
  • Species
    Synthetic
  • Insert Size (bp)
    234
  • Promoter 5S
  • Tag / Fusion Protein
    • 5S rRNA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Seq-pAV-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV5S-F30-2xdBroccoli was a gift from Samie Jaffrey (Addgene plasmid # 66845 ; http://n2t.net/addgene:66845 ; RRID:Addgene_66845)