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gh76
(Plasmid #106747)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106747 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MGAT1
  • gRNA/shRNA sequence
    CCCTCAGTCAGCGCTCTCGA
  • Species
    H. sapiens (human)
  • Entrez Gene
    MGAT1 (a.k.a. GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, GnTI, MGAT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer QCG forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gh76 was a gift from Eric-Paul Bennett (Addgene plasmid # 106747 ; http://n2t.net/addgene:106747 ; RRID:Addgene_106747)
  • For your References section:

    A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall HH. Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101. 10.1093/glycob/cwx101 PubMed 29315387