Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall HH
Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101. PubMed Article

More than 200 glycosyltransferases are involved in biosynthesis of the human glycome, which is comprised of all glycan structures found on different glycoconjugates in all cells. In short of the necessary analytic strategies that enable researchers to decipher the biological roles of any glycan with respect to structure and/or function, the biosynthetic roles of most of these enzymes have been defined. Thus, GlycoCRISPR provides the first collection of CRISPR/Cas9 gRNAs targeting all human glycosyltransferase encoded genes (glycogenes). The uniqueness of this collection becomes evident from the fact that all gRNA designs deposited in the collection have been experimentally validated by recently published methods (Lonowski et al., 2017) to give rise to >30% target cutting efficiency based on in depth indel profiling by IDAA (Yang et al., 2015). GlycoCRISPR gRNAs are made available as a sustainable plasmid format encoding U6 promoter driven gRNA expression. The promised high indel formation capacity of all glycogene gRNA designs enables easy genetic dissection of glycan biosynthetic pathways either as single and/or multiplex combinatorial targeting of the human glycosyltransferase genome.
Workflow for establishment of the GlycoCRISPR collection 205.3Â KBAdditional References
- Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frödin M. 2017. Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis. Nat Protoc. Mar;12(3):581-603. PMID: 28207001
- Yang Z, Steentoft C, Hauge C, Hansen L, Thomsen AL, Niola F, Vester-Christensen MB, Frödin M, Clausen H, Wandall HH, Bennett EP. 2015. Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res. May 19;43(9):e59. PMID: 25753669
Plasmids from Article
ID | Plasmid | Purpose | |
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106682 | gh1 | expresssion of gRNA targeting GALNT1 |
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106683 | gh10 | expresssion of gRNA targeting GALNT10 |
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106684 | gh11 | expresssion of gRNA targeting GALNT11 |
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106685 | gh12 | expresssion of gRNA targeting GALNT12 |
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106686 | gh2 | expresssion of gRNA targeting GALNT2 |
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106687 | gh3 | expresssion of gRNA targeting GALNT3 |
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106688 | gh4 | expresssion of gRNA targeting GALNT4 |
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106689 | gh5 | expresssion of gRNA targeting GALNT5 |
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106690 | gh6 | expresssion of gRNA targeting GALNT6 |
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106692 | gh8 | expresssion of gRNA targeting GALNT8 |
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106693 | gh9 | expresssion of gRNA targeting GALNT9 |
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106694 | gh13 | expresssion of gRNA targeting GALNT13 |
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106695 | gh22 | expresssion of gRNA targeting COSMC |
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106696 | gh23 | expresssion of gRNA targeting POMT1, Cas9, and GFP |
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106697 | gh24 | expresssion of gRNA targeting POMT2 |
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106698 | gh14 | expresssion of gRNA targeting GALNT14 |
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106699 | gh15 | expresssion of gRNA targeting GALNT15 |
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106700 | gh16 | expression of gRNA targeting GALNT16 |
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106701 | gh17 | expresssion of gRNA targeting GALNT17 |
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106702 | gh18 | expresssion of gRNA targeting GALNT18 |
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106703 | gh19 | expresssion of gRNA targeting GALNT19 |
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106704 | gh20 | expresssion of gRNA targeting GALNT20 |
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106705 | gh21 | expresssion of gRNA targeting C1GALT1 |
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106706 | gh25 | expresssion of gRNA targeting POMGNT1 |
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106707 | gh44 | expresssion of gRNA targeting ST6GALNAC3 |
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106708 | gh45 | expresssion of gRNA targeting ST6GALNAC4 |
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106709 | gh46 | expresssion of gRNA targeting ST6GALNAC5 |
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106710 | gh33 | expresssion of gRNA targeting B4GALNT3 |
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106711 | gh34 | expresssion of gRNA targeting B4GALNT4 |
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106712 | gh38 | expresssion of gRNA targeting STT3A |
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106713 | gh39 | expresssion of gRNA targeting STT3B |
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106714 | gh40 | expresssion of gRNA targeting ST6GAL1 |
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106715 | gh41 | expresssion of gRNA targeting ST6GAL2 |
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106716 | gh42 | expresssion of gRNA targeting ST6GALNAC1 |
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106717 | gh43 | expresssion of gRNA targeting ST6GALNAC2 |
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106718 | gh47 | expresssion of gRNA targeting ST6GALNAC6 |
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106720 | gh60 | expresssion of gRNA targeting B3GAT3 |
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106722 | gh49 | expresssion of gRNA targeting ST3GAL1 |
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106723 | gh50 | expresssion of gRNA targeting ST3GAL2 |
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106724 | gh51 | expresssion of gRNA targeting ST3GAL3 |
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106725 | gh52 | expresssion of gRNA targeting ST3GAL4 |
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106726 | gh53 | expresssion of gRNA targeting ST3GAL5 |
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106727 | gh54 | expresssion of gRNA targeting ST3GAL6 |
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106728 | gh55 | expresssion of gRNA targeting B4GALT7 |
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106730 | gh62 | expresssion of gRNA targeting B4GALT2 |
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106731 | gh71 | expresssion of gRNA targeting B3GNT5 |
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106732 | gh72 | expresssion of gRNA targeting B3GNT6 |
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106733 | gh73 | expresssion of gRNA targeting B3GNT7 |
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106734 | gh63 | expresssion of gRNA targeting B4GALT3 |
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106735 | gh64 | expression of gRNA targeting B4GALT4 |
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106736 | gh65 | expresssion of gRNA targeting B4GALT5 |
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106737 | gh66 | expresssion of gRNA targeting B4GALT6 |
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106738 | gh67 | expresssion of gRNA targeting B3GNT1 (B4GAT1) |
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106739 | gh68 | expresssion of gRNA targeting B3GNT2 |
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106740 | gh69 | expresssion of gRNA targeting B3GNT3 |
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106741 | gh70 | expresssion of gRNA targeting B3GNT4 |
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106742 | gh74 | expresssion of gRNA targeting B3GNT8 |
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106743 | gh83 | expresssion of gRNA targeting MGAT5 |
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106744 | gh84 | expresssion of gRNA targeting MGAT5B |
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106745 | gh85 | expresssion of gRNA targeting FUT1 |
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106746 | gh75 | expresssion of gRNA targeting B3GNT9 |
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106747 | gh76 | expresssion of gRNA targeting MGAT1 |
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106748 | gh77 | expresssion of gRNA targeting MGAT2 |
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106749 | gh78 | expresssion of gRNA targeting MGAT3 |
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106750 | gh79 | expresssion of gRNA targeting MGAT4A |
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106751 | gh80 | expresssion of gRNA targeting MGAT4B |
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106752 | gh81 | expresssion of gRNA targeting MGAT4C |
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106753 | gh82 | expresssion of gRNA targeting MGAT4D |
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106754 | gh86 | expresssion of gRNA targeting FUT2 |
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106755 | gh95 | expresssion of gRNA targeting FUT11 |
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106756 | gh96 | expresssion of gRNA targeting POFUT1 |
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106757 | gh97 | expresssion of gRNA targeting POFUT2 |
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106758 | gh87 | expresssion of gRNA targeting FUT3 |
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106759 | gh88 | expresssion of gRNA targeting FUT4 |
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106760 | gh89 | expresssion of gRNA targeting FUT5 |
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106761 | gh90 | expresssion of gRNA targeting FUT6 |
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106762 | gh91 | expresssion of gRNA targeting FUT7 |
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106763 | gh92 | expresssion of gRNA targeting FUT8 |
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106764 | gh93 | expresssion of gRNA targeting FUT9 |
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106765 | gh94 | expresssion of gRNA targeting FUT10 |
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106766 | gh98 | expresssion of gRNA targeting LFNG |
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106767 | gh108 | expresssion of gRNA targeting B3GALT5 |
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106768 | gh109 | expresssion of gRNA targeting B3GALT6 |
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106769 | gh110 | expresssion of gRNA targeting B3GALTL (B3GLCT) |
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106770 | gh99 | expresssion of gRNA targeting MFNG |
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106771 | gh100 | expresssion of gRNA targeting RFNG |
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106772 | gh101 | expresssion of gRNA targeting POGLUT1 |
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106773 | gh103 | expresssion of gRNA targeting GXYLT2 |
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106774 | gh104 | expresssion of gRNA targeting XXYLT1 |
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106775 | gh105 | expresssion of gRNA targeting B3GALT1 |
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106776 | gh106 | expresssion of gRNA targeting B3GALT2 |
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106777 | gh107 | expresssion of gRNA targeting B3GALT4 |
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106778 | gh111 | expresssion of gRNA targeting B3GALNT1 |
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106779 | gh120 | expresssion of gRNA targeting GCNT7 |
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106780 | gh121 | expresssion of gRNA targeting ST8SIA1 |
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106781 | gh122 | expresssion of gRNA targeting ST8SIA2 |
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106782 | gh112 | expresssion of gRNA targeting B3GALNT2 |
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106783 | gh113 | expresssion of gRNA targeting B4GALNT1 |
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106784 | gh114 | expresssion of gRNA targeting B4GALNT2 |
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106785 | gh115 | expresssion of gRNA targeting GCNT1 |
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106786 | gh116 | expression of gRNA targeting GCNT2 |
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106787 | gh117 | expresssion of gRNA targeting GCNT3 |
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106788 | gh118 | expresssion of gRNA targeting GCNT4 |
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106789 | gh119 | expresssion of gRNA targeting GCNT6 |
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106790 | gh123 | expresssion of gRNA targeting ST8SIA3 |
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106791 | gh132 | expresssion of gRNA targeting CHPF2 |
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106792 | gh133 | expresssion of gRNA targeting CHSY1 |
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106793 | gh134 | expresssion of gRNA targeting CHSY3 |
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106794 | gh124 | expresssion of gRNA targeting ST8SIA4 |
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106795 | gh125 | expresssion of gRNA targeting ST8SIA5 |
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106796 | gh126 | expresssion of gRNA targeting ST8SIA6 |
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106797 | gh127 | expresssion of gRNA targeting XYLT1 |
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106798 | gh128 | expresssion of gRNA targeting XYLT2 |
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106799 | gh129 | expresssion of gRNA targeting CSGALNACT1 |
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106800 | gh130 | expresssion of gRNA targeting CSGALNACT2 |
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106801 | gh131 | expresssion of gRNA targeting CHPF |
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106802 | gh135 | expresssion of gRNA targeting EXT1 |
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106803 | gh144 | expresssion of gRNA targeting LARGE |
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106804 | gh145 | expresssion of gRNA targeting FKRP |
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106805 | gh146 | expresssion of gRNA targeting FKTN |
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106806 | gh136 | expresssion of gRNA targeting EXT2 |
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106807 | gh137 | expresssion of gRNA targeting EXTL1 |
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106808 | gh138 | expresssion of gRNA targeting EXTL2 |
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106809 | gh139 | expresssion of gRNA targeting EXTL3 |
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106810 | gh140 | expresssion of gRNA targeting GBGT1 |
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106811 | gh141 | expresssion of gRNA targeting OGT |
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106812 | gh142 | expresssion of gRNA targeting EOGT |
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106813 | gh143 | expresssion of gRNA targeting POMGNT2 |
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106814 | gh147 | expresssion of gRNA targeting KDELC1 |
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106815 | gh162 | expresssion of gRNA targeting TMTC3 |
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106816 | gh163 | expresssion of gRNA targeting TMTC4 |
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106817 | gh166 | expresssion of gRNA targeting A3GALT2 |
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106818 | gh148 | expresssion of gRNA targeting KDELC2 |
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106819 | gh149 | expresssion of gRNA targeting GLT1D1 |
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106820 | gh150 | expresssion of gRNA targeting GTDC1 |
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106821 | gh151 | expresssion of gRNA targeting GLT8D1 |
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106822 | gh152 | expresssion of gRNA targeting GLT8D2 |
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106823 | gh156 | expresssion of gRNA targeting GYLT1B (LARGE2) |
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106824 | gh160 | expresssion of gRNA targeting TMTC1 |
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106825 | gh161 | expresssion of gRNA targeting TMTC2 |
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106826 | gh167 | expresssion of gRNA targeting A4GALT |
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106827 | gh177 | expresssion of gRNA targeting HAS3 |
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106828 | gh178 | expresssion of gRNA targeting GYS1 |
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106830 | gh168 | expresssion of gRNA targeting A4GNT |
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106831 | gh169 | expresssion of gRNA targeting ABO |
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106832 | gh171 | expresssion of gRNA targeting UGCG |
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106833 | gh172 | expresssion of gRNA targeting UGGT1 |
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106834 | gh173 | expresssion of gRNA targeting UGGT2 |
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106835 | gh174 | expresssion of gRNA targeting UGT8 |
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106836 | gh175 | expresssion of gRNA targeting HAS1 |
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106837 | gh176 | expresssion of gRNA targeting HAS2 |
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106838 | gh180 | expresssion of gRNA targeting GYG1 |
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106839 | gh236 | expresssion of gRNA targeting DPY19L3 |
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106840 | gh237 | expresssion of gRNA targeting DPY19L4 |
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106841 | gh238 | expresssion of gRNA targeting DPM1 |
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106842 | gh181 | expresssion of gRNA targeting GYG2 |
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106843 | gh182 | expresssion of gRNA targeting GLT6D1 |
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106844 | gh183 | expresssion of gRNA targeting CERCAM |
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106845 | gh184 | expresssion of gRNA targeting COLGALT1 |
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106846 | gh185 | expresssion of gRNA targeting COLGALT2 |
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106847 | gh186 | expresssion of gRNA targeting B3GNTL1 |
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106848 | gh234 | expresssion of gRNA targeting DPY19L1 |
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106849 | gh235 | expresssion of gRNA targeting DPY19L2 |
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106850 | gh242 | expresssion of gRNA targeting PIGA |
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106851 | gh260 | expresssion of gRNA targeting ALG6 |
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106852 | gh261 | expresssion of gRNA targeting ALG8 |
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106853 | gh262 | expresssion of gRNA targeting ALG9 |
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106854 | gh243 | expresssion of gRNA targeting PIGB |
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106855 | gh246 | expresssion of gRNA targeting PIGM |
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106856 | gh249 | expresssion of gRNA targeting PIGV |
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106857 | gh250 | expresssion of gRNA targeting PIGZ |
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106859 | gh257 | expresssion of gRNA targeting ALG2 |
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106860 | gh258 | expresssion of gRNA targeting ALG3 |
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106861 | gh259 | expresssion of gRNA targeting ALG5 |
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106862 | gh263 | expresssion of gRNA targeting ALG10 |
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106863 | gh264 | expresssion of gRNA targeting ALG10B |
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106864 | gh265 | expresssion of gRNA targeting ALG11 |
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106865 | gh266 | expresssion of gRNA targeting ALG12 |
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106866 | gh267 | expresssion of gRNA targeting ALG13 |
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106867 | gh268 | expresssion of gRNA targeting ALG14 |
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