416 pSG5L HA RB del22
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10721||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRB del22
SpeciesH. sapiens (human)
Insert Size (bp)2800
MutationDeletion of exon 22 (aa738-775)
Entrez GeneRB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, pRb, pp110)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
EcoRI is an internal site as well. See author's map. To learn more about del22 mutant, see reference: Sellers WR, Rodgers JW, and Kaelin WG, PNAS 92:11544.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:416 pSG5L HA RB del22 was a gift from William Sellers (Addgene plasmid # 10721 ; http://n2t.net/addgene:10721 ; RRID:Addgene_10721)
For your References section:Stable binding to E2F is not required for the retinoblastoma protein to activate transcription, promote differentiation, and suppress tumor cell growth. Sellers WR, Novitch BG, Miyake S, Heith A, Otterson GA, Kaye FJ, Lassar AB, Kaelin WG. Genes Dev. 1998 Jan 1. 12(1):95-106. 10.1101/gad.12.1.95 PubMed 9420334
Map uploaded by the depositor.