GW1-mito-roGFP1-mApple
(Plasmid
#107218)
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PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.
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Depositing Lab
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Depositing OrganizationPurdue University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 107218-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 107218-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneGW1-mito
- Backbone size w/o insert (bp) 5649
- Total vector size (bp) 7137
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameroGFP1-mApple
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Insert Size (bp)1488
- Promoter CMV
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Tag
/ Fusion Protein
- 4xCox8 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SgrAI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CTG AAT CGG AGT GAG TGC
- 3′ sequencing primer CCT CTA CAA ATG TGG TAT GG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 107218-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 107218-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GW1-mito-roGFP1-mApple was a gift from Mathew Tantama (Addgene plasmid # 107218 ; http://n2t.net/addgene:107218 ; RRID:Addgene_107218) -
For your References section:
Extending roGFP Emission via Forster-Type Resonance Energy Transfer Relay Enables Simultaneous Dual Compartment Ratiometric Redox Imaging in Live Cells. Norcross S, Trull KJ, Snaider J, Doan S, Tat K, Huang L, Tantama M. ACS Sens. 2017 Nov 22;2(11):1721-1729. doi: 10.1021/acssensors.7b00689. Epub 2017 Nov 7. 10.1021/acssensors.7b00689 PubMed 29072071
Map uploaded by the depositor.
